【摘要】 采用细胞倍增时间、分裂指数、生化变化和软琼脂克隆形成率等指标测定分化 ,研究了抗坏血酸和亚硒酸钠对人肝癌细胞逆转的作用 ..结果表明 :用抗坏血酸 4mmol·L- 1 联合亚硒酸钠 2 μmol·L- 1 处理细胞后 ,细胞的生长率和有丝分裂指数都显著下降 .一些与细胞恶性表型有关的指标 ,如细胞表面电荷明显下降 ,电泳率从 1.74 μm·s- 1 ·V- 1 ·cm- 1 下降到 0 .91μm·s- 1 ·V- 1 ·un- 1 ,甲胎蛋白含量从 331μg·g- 1 (蛋白 )下降到 90 μg·g- 1 ,γ 谷氨酰转肽酶活性从 0 .74U·g- 1 (蛋白 )下降到 0 .19U·g- 1 ..与细胞分化有关的指标 ,如酪氨酸 α 酮戊二酸转氨酶 (TAT)的活性从 14.7μmol·g- 1 (蛋白 )升高至 4 2 .6 μmol·g- 1 ,软琼脂克隆形成能力下降 94 .7% .由此可见 ,抗坏血酸和亚硒酸钠联合能诱导肝癌细胞在分化 ,逆转肿瘤细胞的恶性表型 .更多还原

【Abstract】 To study the effects of sodium selenite on growth inhibition and differentiation in human hepatoma cells in culture.. Cells growth inhibition, biological changes and cloning efficiencies in soft agar are measured with ascorbic acid (AA) 6 mmol·L -1 as a positive control.. The results show that after treatment with Ascorbic acid 4 mmol·L -1 and sodium selenite 2 μmol·L -1 , the growth rate and mitotic index of human hepatoma cells decrease remarkably. The indexes related with cell malignancy are alleviated,e.g. cell surface charge obviously decreases, the electrophresis rate dropps from 1.74 μm·s -1 ·V -1 ·cm -1 to 0 91 μm·s -1 ·V -1 ·cm -1 , the average content of α fetoprotein (α FP) decreases from 331 μg·g -1 (protein) to 90 μg·g -1 , and γ glutamyl transpeptidase(γ GT) activity drops from 0.74 U·g -1 (protein) to 0 19 U·g -1 . The index related with cell differentiation increases, such as the level of tyrosine α ketoglutarate transaminase(TAT) activity increases from 14.7 μmol·g -1 (protein) to 42.6 μmol·g -1 ,and the colonogenic potential decreases by 94..7 %. It is clear that the combination ascorbic acid sodium selenite can inhibit human hepatoma cells proliferation, induce redifferenitiation, and reverse its malignant phenotypic characteristics.更多还原