【摘要】 目的　探讨脑梗死急性期血小板GMP - 140、血浆GMP - 140、血小板粘附率和聚集率的变化及意义。方法　对大灶脑梗死组 (n =2 2 )、小灶脑梗死组 (n =2 5 )和腔隙性脑梗死组 (n =2 0 )病人进行研究 ,于发病后 3d和 2周采静脉血 ,采用酶联免疫吸附竞争法测定血小板GMP - 140、酶联免疫吸附双抗体夹心法测定血浆GMP - 140的浓度。结果　发病 3d内大灶脑梗死组血小板GMP - 140、血浆GMP - 140浓度较对照组非常显著升高 (P <0 .0 0 1) ,小灶脑梗死组和腔隙性脑梗死组血小板GMP - 140、血浆GMP - 140浓度也较对照组升高 (P <0 .0 0 5～0 .0 0 1) ,但无大灶脑梗死组升高明显 (P <0 .0 5 ) ;小灶脑梗死组与腔隙性脑梗死组无显著性差异 (P >0 .0 5 ) ;3组病人血小板粘附率 (PAdT)和血小板聚集率 (PAgT)均显著升高 (P <0 .0 1)。发病 2周时 ,3组病人血小板GMP -140、血浆GMP - 140浓度均已明显下降 ,但仍较对照组高 (P <0 .0 5 ) ,而PAdT和PAgT均降至正常或更低。结论　不同类型脑梗死急性期血小板处于显著的活化状态 ,血浆GMP - 140浓度PAdT和PAgT能更准确地反映血小板的活化状态。更多还原

【Abstract】 Objective\ To explore the changes and significances of platelet granule membrane protein-140 (GMP-140),GMP-140 in plasma,PAdT and PAgT in patients with acute cerebral infarction.Methods\ The platelet GMP-140 was measured with enzyme-linked immunosorbent assay (ELISA) competitive method and GMP-140 in plasma by ELISA double antibody method in blood collecting in 3 d and 2 weeks after onset in large(n=22),small(n=25) size and lacuna (n=20) cerebral infarction groups.Results\ The platelet GMP-140,GMP-140 in plasma,PAdT and PAgT in large cerebral infarction group in 3 d after onset were much higher than control group (P<0.001),and the small size and lacuna groups were higher (P<0.05～0.001) than control but lower than large group (P<0.05).There was no differences between the small and lacuna groups (P>0.05).The PAdT and PAgT in 3 groups were higher (P<0.01).The platelet GMP-140 and GMP-140 in plasma in all 3 groups in 2 weeks after onset had been clearly lower but still higher than control group (P<0.05),the PAdT and PAgT were normal or even more lower.Conclusion\ Platelet activation was significant in different types acute cerebral infarction,the concentration of GMP-140 in plasma can reflect the degree of platelets activities more well and truly than PAdT and PAgT.更多还原