【摘要】 重组人粒细胞 -巨噬细胞集落刺激因子 (rHuGM -CSF)表达产物在大肠杆菌中以包涵体的形式存在。方法　包涵体高压匀浆破菌抽提后 ,经凝胶过滤层析、复性、离子交换层析及凝胶过滤层析等纯化步骤。结果终产物纯度达 97% ,比活性达 1 2× 10 7U/mg蛋白 ,测定N端 2 0个氨基酸系列与其DNA系列推导的氨基酸系列完全一致。更多还原

【Abstract】 The recombinant human granulocyte-macrphage colony stimulating factor (rHuGM-CSF)exressed in Esherichia coli exists in the form of insoluble inclusion bodies.The inclusion bodies were separated form cytoplasm by homogenization.They were purified by gel filtration chromatography,renaturation,and ion exchange.The purity of final product reached 97%,and the specific activity reached 1 2×10U/mg protein.Its amino acid composition and partial NH 2-terminal sequence(up to twenty residues)were also identical with its cDNA sequence reported for this protein.更多还原