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木霉菌对烟草黑胫病菌的拮抗机制及其生物防治研究

Study on the Antaganistic Mechanism of Trichoderma sp.for Phytophthora Nicotianae and it’s Biocontrol Effection

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【作者】 王革李梅云段玉琪李松马永凯王颖琦朱维明李振国李天飞

【Author】 WANG Ge 1, LI Mei yun 2, DUAN Yu qi 2, LI Song 1, MA Yong kai 1,WANG Ying qi 1, ZHU Wei ming 1, LI Zheng guo 1, LI Tian fei 2 (1.Research and Development Center,Hongta Group,Yuxi 653100,China; 2.Agricultural Institute,Yunnan Academy of T

【机构】 云南玉溪红塔集团技术中心!云南玉溪653100云南烟草科学研究院农业所!云南玉溪653100云南玉溪红塔集?

【摘要】 利用诱捕法从土壤中分离得到 1株木霉菌株长枝木霉 (Trichodermalongibrachiatum ) ,采用对峙法测定其对云南省烟草黑胫病菌 (Phytophthoranicotiana)的拮抗作用 ,结果表明 :该菌对黑胫病菌具较强拮抗作用 ,拮抗机制主要为竞争作用、重寄生作用、抗生作用 .采用温室盆栽、田间试验 ,分别测定该菌对黑胫病的防效 ,室内防效为 97.6 % (10 6个 /mL浓度级 ) ;田间移栽时 ,每株施用 4g效果较好 ,田间防效可达 6 7%以上 .

【Abstract】 One isolate of Trichoderma sp.was obtained by the method of baiting.The antagonistic test of the isolate to strains of Phtophthora nicotiana (pathogen of tobacco black shank) from diseased tobacco stems from different area of Yunnan were carried out by pairing cuture on PDA.The results showed that this isolate was strong antagonistic to black shank fungus.The antagonistic mechanism appeared to be competitive growth and antibiosis.The control effect of this isolate applied for tobacco black shank in greenhouse was as high as 97.6%(10 6?cfu/mL).When applied in field soil around the tobacco root at 4?g every root,the control effect could arrive at 67%.

  • 【文献出处】 云南大学学报(自然科学版) ,Journal of Yunnan University (Natural Sciences) , 编辑部邮箱 ,2001年03期
  • 【分类号】S476.7
  • 【被引频次】145
  • 【下载频次】489
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