【摘要】 目的 探讨白细胞介素－１β（ＩＬ－１β）对大鼠视网膜单核细胞趋化蛋白（ＭＣＰ）－１ｍＲＮＡ表达的诱导作用。方法 ２４只ＳＤ大鼠，右眼为实验眼，左眼为对照眼。实验眼玻璃体内注入ＩＬ－１β ２５０ Ｕ／５μｌ，对照眼注入等量生理盐水（ＰＳＳ）。注射后４，２４ｈ取视网膜组织，提取总ＲＮＡ应用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测视网膜ＭＣＰ－１ｍＲＮＡ表达。结果 眼内ＩＬ－１β注射后４ｈ实验眼视网膜ＭＣＰ－１ｍＲＮＡ表达水平较对照眼明显增加；２４ｈ实验眼视网膜ＭＣＰ－１ｍＲＮＡ水平与对照眼相近。结论 视网膜ＭＣＰ－１ｍＲＮＡ可被眼内ＩＬ－１β诱导表达，ＭＣＰ－１可能在ＩＬ－１β诱发的眼内炎症反应中发挥作用。更多还原

【Abstract】 Objective To evaluate the inductive role interleukin(IL) -1β on the expression of monocyte chemotactic pro- tein(MCP) -1 mRNA in retina in rat. Methods twenty-four SD rats were studied by injecting intravitreally IL-1β 250 U/5 μl in the right(experimental) eyes and PSS 5 μl in the left(control) eyes. Total RNA was isolated from the retina 4 h and 24 h post-IL-1β challenge,and reverse-transcription-polymeras chain reaction (RT-PCR) was used to examine the expression of MCP-1 mRNA in the retina. Results Expression of MCP-1 mRNA increased remarkably in retina of the experimental eyes 4 hours post-IL-1β challenge,and returned to a decreased level similar to that of the control eyes at 24 hours. Conclusion Expression of MCP-1 mRNA in retina can be induced by intraocular IL-1β. MCP-1 may play a role in IL-1β-induced intraocular inflammation.更多还原