【摘要】 目的　　探索用准分子激光切削技术制备视网膜单层视细胞植片，经内入路视网膜下腔的单层视细胞成片移植。方法用准分子激光对大鼠视网膜进行切削，制取单层视细胞植片，此后，按内入路手术方法进行了兔视网膜下腔的异种移植。结果　切削后所得视细胞植片由单层视细胞组成，结构完整，包括外丛状层、外核层和外节层；视细胞植片经明胶包埋后被准确植入宿主视网膜下腔中，移植术后第１，２天宿主视网膜未能复位，呈脱离状态，移植物没能与视网膜色素上皮层相贴；移植后１０天，宿主视网膜复位，视细胞移植片平铺于宿主视网膜下腔中，植片视细胞外节与宿主视网膜色素上皮层相贴，未见明显免疫排异现象。结论　　准分子激光制备单层视细胞植片方法简单、可行；初步观察到内入路单层视细胞成片移植后，视细胞植片能够在宿主视网膜下腔中以正常生理位置存活；视网膜下腔为理想的视网膜移植的受位。更多还原

【Abstract】 Objective To explore the preparation of monolayer photoreceptor sheet via excimer laser ablation and transplantation of monolayer photoreceptor sheet into subretinal space through intraocular approach. Methods Ablation of rat retinae was performed by excimer laser and monolayer photoreceptor sheets were obtained. And then the monolayer photoreceptor sheets from rats were transplanted into rabbit subretinal spaces according to internal-operation approach procedure. Results All paris of the photoreceptor cell in the sheet maintained intact, including the outer segments, inner segments, outer limiting membrane, outer nuclear layer, and outer plexiform layer. The heterogeneous photoreceptor sheet supported by gelatin was inserted and well positioned into the subretinal space between the recipient’s photoreceptor and RPE cell layers. 1 day and 2 day after transplantation surgery,recipient retina retained detachment and donor transplant did not keep well close to recipient RPE layer. 10 day after surgery, the donor photoreceptor sheet spread out in the subretinal space and integrated with host cells. The immune rejection inflammation was not found in all of retina recieved transplantation. Conclusions The preparation of excimer laser ablation for photoreceptor sheet is simple and applicable. The heterogeneous donor photoreceptor sheet can survive in host subretinal space. The subretinal space may be an ideal recipient site for retinal transplantation.更多还原