【摘要】 目的　探讨纯培养神经元和星形胶质细胞的合适方法。方法　采用Balb/c鼠尾胶原、大鼠尾胶原、阿糖胞苷、贴壁处理及传代培养等方法纯培养Balb/c鼠神经元和星形胶质细胞。结果　纯培养Balb/c鼠神经元的纯度为 90 % ,纯培养星形胶质细胞的纯度为 97%。结论　纯培养神经元宜选择Balb/c鼠尾胶原为生长基质并经 3mg·L-1阿糖胞苷处理。纯培养星形胶质细胞宜用大鼠尾胶原为生长基质和培养 10天左右采用二次差速贴壁处理及传代培养更多还原

【Abstract】 Objective To seek suitable methods of neuron culture and a strocytes culture from newborn Balb/cmouse brain cortex. Methods Balb/c mouse tail collagen, rat tail collagen, cytosine arabinoside; adhesion treatment and subculture were adopted to culture neurons and astrocytes. Results The purity of neuron cultures was 90 percent, whereas the purity of astrocyte cultures was 97 percent. Conclusion Balb/c mouse tail collagen and cytosine arabinoside may be applied to culture neurons, whereas rat tail collagen, adhesion treatment and subculture may be applied to culture astrocytes.更多还原