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大鼠部分肝切除前后热激处理对过氧化物酶基因表达的影响

Expression Difference of the Peroxidase Genes in Partial Hepatectomy and Heat Shock

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【作者】 徐存拴张为民王莹赵绪永

【Author】 XU Cun-shuan 1,ZHANG Wei-min 1,WANG Ying 2 ,ZHAO Xu-yong 1 (1.College of Life Sciences,Henan Normal University,Xinxiang,Henan 453002,China; 2.College of Life Science and Technology,Jinan University,Guangzhou 510632,China)

【机构】 河南师范大学生命科学学院暨南大学生命科学技术学院河南师范大学生命科学学院 河南新乡453002河南新乡453002广州510632河南新乡453002

【摘要】 用过氧化物酶原位复性电泳 (SDS -POD -PAGE)技术分析了部分肝切除 (partialhepatectomy ,PH)、部分肝切除后再热激 (partialhepatectomyfollowingheatshock ,PH -HS)和先热激再部分肝切除 (heatshockfollowingpartialhepate ctomy ,HS -PH)后肝再生期间过氧化物酶 (POD)基因表达差异 ,结果表明 ,PH中表达的POD基因种类 (7个 ) >PH-HS(5个 ) >HS -PH(3个 ) ;三个模型的POD总活性为 :HS -PH >PH >PH -HS。根据实验结果推测 ,POD在肝再生和肝损伤恢复中起一定作用。

【Abstract】 Expression difference of the peroxidase (POD) genes during liver regeneration after partial hepatectomy (PH), partial hepatectomy following heat shock (PH-HS) and heat shock following partial hepatectomy (HS-PH) was analyzed by POD renatured electrophoresis (SDS-POD-PAGE). The results showed that the expressed POD gene kinds in PH, PH-HS and HS-PH were 7, 5 and 3 respectively, and that the total activities of POD in the three models were HS-PH > PH > PH-HS. The results suggested that POD play some roles during liver regeneration and in recovery after liver damage.

【基金】 河南省生物工程重点实验室资助项目 (编号 :PKL990 0 2 )
  • 【分类号】Q344.13
  • 【被引频次】2
  • 【下载频次】38
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