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大鼠部分肝切除前后热激处理对过氧化物酶基因表达的影响
Expression Difference of the Peroxidase Genes in Partial Hepatectomy and Heat Shock
【摘要】 用过氧化物酶原位复性电泳 (SDS -POD -PAGE)技术分析了部分肝切除 (partialhepatectomy ,PH)、部分肝切除后再热激 (partialhepatectomyfollowingheatshock ,PH -HS)和先热激再部分肝切除 (heatshockfollowingpartialhepate ctomy ,HS -PH)后肝再生期间过氧化物酶 (POD)基因表达差异 ,结果表明 ,PH中表达的POD基因种类 (7个 ) >PH-HS(5个 ) >HS -PH(3个 ) ;三个模型的POD总活性为 :HS -PH >PH >PH -HS。根据实验结果推测 ,POD在肝再生和肝损伤恢复中起一定作用。
【Abstract】 Expression difference of the peroxidase (POD) genes during liver regeneration after partial hepatectomy (PH), partial hepatectomy following heat shock (PH-HS) and heat shock following partial hepatectomy (HS-PH) was analyzed by POD renatured electrophoresis (SDS-POD-PAGE). The results showed that the expressed POD gene kinds in PH, PH-HS and HS-PH were 7, 5 and 3 respectively, and that the total activities of POD in the three models were HS-PH > PH > PH-HS. The results suggested that POD play some roles during liver regeneration and in recovery after liver damage.
【Key words】 partial hepatectomy (PH); heat shock (HS); peroxidase (POD); allelic enzyme; induced enzyme; liver regeneration;
- 【文献出处】 遗传 ,Hereditas(Beijing) , 编辑部邮箱 ,2001年06期
- 【分类号】Q344.13
- 【被引频次】2
- 【下载频次】38