【摘要】 总结了十多年来我们从事ES细胞建系和培养工作的经验和教训 ,提出了研究工作中存在的问题以及解决这些问题的方法和对策 ,并对一些常规性的操作步骤进行了改进。同时 ,对某些尚不明确的问题进行了讨论 ,提出了我们的看法和建议。研究工作表明 ,我们采取的对策和方法是可行、有效的更多还原

【Abstract】 Based on manipulating Embryonic Stem cells for more than ten years, we proposed our methods to solve the problems in establishing and maintaining ES cell lines, especially the improvement of routine operational approach.We also discussed some ambiguous problems and gave corresponding opinion and suggestion which was proved to be feasible and effective in our own research.更多还原