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一种改良的鱼类染色体富集制片方法
An Improved Technique of EnrichedFish Chromosome Preparations
【摘要】 运用淋巴细胞分离液处理黄鳝肾脏细胞悬浮液后,成功地分离了其中红细胞和淋巴细胞。以富集的淋巴细胞进行染色体制片,可避免有核红细胞的影响,获得高分裂指数的黄鳝染色体标本。该方法简便、结果稳定, 亦适用于其他鱼类、两栖类和鸟类的染色体制片。
【Abstract】 After treated by the lymphocyte separation medium, the erythrocytes and lymphocytes were separated successfully in the kidney cells suspension of rice field eel. Using the enriched lymphocytes to prepare chromos ome, we get the high split-index chromosome preparations without the interferenc e of the erythrocytes. This technique is simple and convenient to prepare the ch romosome of other fishes or other animals for FISH.
【关键词】 黄鳝;
淋巴细胞分离;
染色体制片;
【Key words】 rice field eel; separation of lymphocytes; chromosome preparation;
【Key words】 rice field eel; separation of lymphocytes; chromosome preparation;
- 【文献出处】 遗传 ,Hereditas(Beijing) , 编辑部邮箱 ,2001年02期
- 【分类号】Q343
- 【被引频次】31
- 【下载频次】279