【摘要】 根据位于小麦第四同源群上与耐盐有关的ｇｆ－２．８基因的编码区序列设计１对引物，分别以两个耐盐突变体及其亲本的总ＤＮＡ为模板进行ＰＣＲ扩增，在５个供试材料中均扩增出１条约６８５ｂｐ的目的条带。ＳＳＣＰ电泳显示突变体９７４９１５与其他供试材料之间存在差异。测序表明冀麦２４和其耐盐突变体８９０１－１７的扩增产物序列与ｇｆ－２．８基因的发表序列相同，这表明突变体８９０１－１７的突变位点不在该基因上；而另一耐盐突变体９７４９１５的序列中则至少存在２个单碱基突变，有一处突变导致了氨基酸的变化，该突变位点位于ｇｆ－２．８基因的保守区域内。更多还原

【Abstract】 gf-2.8 is a gene located on the chromosomal homologous group 4, which was reported to have relationship with salt tolerance of wheat. A pair of primers were designed to amplify the coding region of gf-2.8 in the two salt- tolerant mutants and their parents. Agarose gel electrophoresis showed that the 685bp band was amplified among all the materials. SSCP analysis suggested that 974915 (one of the mutants) was different from the other materials. The sequencing results showed that Jimai 24 and its salt-tolerant mutant 8901-17 bad the same sequence as published, which indicated that the mutation site of 8901- 17 was not on the gf-2.8 gene. However, there were at least two single base mutations in the gf-2.8 of 974915, one of which caused the alternation of amino acid, this mutation occurred in the conservative region of gf-2.8.更多还原