【摘要】 用ＰＣＲ、ＧＵＳ染色和Ｗｅｓｔｅｒｎ点杂交技术检测了Ｂｔ水稻杂交后代群体，在３９４株ＧＵＳ阳性 株中，有３９２株表达Ｂｔ蛋白，协同表达株率达９９．４９％。由此表明，在杂交后代中报告基因ｇｕｓ和 目的基因ｃｒｙ１Ａｂ紧密连锁遗传与表达。ＧＵＳ组织染色和Ｓｏｕｔｈｅｒｎ杂交检测表明Ｂｔ水稻中的 ＣｒｙｌＡｂ基因呈单位点显性基因遗传，且在有性世代中能稳定传递。还发现，在ＢＣ１、ＢＣ１Ｆ２和粳粳 交Ｆ２群体中ＣｒｙｌＡｂ基因呈单位点显性基因遗传，而在籼粳交Ｆ２群体中偏离３∶１分离。更多还原

【Abstract】 Improved histochemical staining for GUS activity, PCR and Western blotting were used to analyse the progeny population of Bt rice crossed with conventional rice varieties. A total of 392 plants expressing Bt toxin protein were found in 394 GUS postive plants. The result demonstrated that crylAb gene closely inherited and expressed with reporter gene gus. GUS assays indicated that crylAb gene of Bt rice inherits as a single dorminant gene. Southern hybridization confirmed crylAb gene can inherit stabily in the progenies of Bt rice. Mendelian segregation of reporter gene gus was also observed in F2, BC1 and BC1F2 progenies, which indicated that crylAb gene inherits as a single dorminant gene in the progenies of Bt rice crossed with conventional rice varieties.更多还原