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Bt水稻中cry1Ab基因的遗传分析

Genetic Analysis of cry1Ab Gene of Bt Rice

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【作者】 王忠华吴刚崔海瑞Illimar Altosaar夏英武舒庆尧

【Author】 WANG Zhong-Hua, WU Gang, CUI Hai-Rui, Illimar Altosaar, XIA Ying-Wu, SHU Qing-Yao (Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; Key Lab of Plant Gene Engineering of Country, Peking University, Beijing 100871,

【机构】 浙江大学原子核农业科学研究所北京大学植物基因工程国家重点实验室加拿大渥太华大学生物化学系浙江大学原子核农业科学研究所 杭州 310029北京100871杭州 310029加拿大渥太华 KIN6N5

【摘要】 用PCR、GUS染色和Western点杂交技术检测了Bt水稻杂交后代群体,在394株GUS阳性 株中,有392株表达Bt蛋白,协同表达株率达99.49%。由此表明,在杂交后代中报告基因gus和 目的基因cry1Ab紧密连锁遗传与表达。GUS组织染色和Southern杂交检测表明Bt水稻中的 CrylAb基因呈单位点显性基因遗传,且在有性世代中能稳定传递。还发现,在BC1、BC1F2和粳粳 交F2群体中CrylAb基因呈单位点显性基因遗传,而在籼粳交F2群体中偏离3∶1分离。

【Abstract】 Improved histochemical staining for GUS activity, PCR and Western blotting were used to analyse the progeny population of Bt rice crossed with conventional rice varieties. A total of 392 plants expressing Bt toxin protein were found in 394 GUS postive plants. The result demonstrated that crylAb gene closely inherited and expressed with reporter gene gus. GUS assays indicated that crylAb gene of Bt rice inherits as a single dorminant gene. Southern hybridization confirmed crylAb gene can inherit stabily in the progenies of Bt rice. Mendelian segregation of reporter gene gus was also observed in F2, BC1 and BC1F2 progenies, which indicated that crylAb gene inherits as a single dorminant gene in the progenies of Bt rice crossed with conventional rice varieties.

【关键词】 Bt水稻cry1Ab基因遗传特点育种
【Key words】 Bt ricecry1Ab genegenetic traitbreeding
【基金】 浙江省科委重大项目(991102004-002);美国洛氏基金资助项目(RF97001#556)
  • 【文献出处】 遗传学报 ,Acta Genetica Sinica , 编辑部邮箱 ,2001年09期
  • 【分类号】S511.03
  • 【被引频次】38
  • 【下载频次】148
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