【摘要】 以９４个Ｏｐｅｒｏｎ公司的１０碱基ＲＡＰＤ引物，对玉米同核同质系（Ⅰ）中的野生型正常可育株４７８（Ｆ）和其突变的不育株４７８－ｃｍｓ的线粒体ＤＮＡ（ｍｔＤＮＡ）进行了 ＲＡＰＤ分析。结果表明：仅ＯＰＺ－１９、ＯＰＡＡ－１５与ＯＰＳ－０１ ３个引物在同核同质系（Ⅰ）的野生型正常可育株和其突变的不育株之间扩增出多态产物ＯＰＺ－１９４２０、ＯＰＡＡ－１５６００与ＯＰＳ－０１４００。提示在同核同质系的野生型正常可育株和其突变的不育株的ｍｔＤＮＡ之间同源性很高，多态性很低，同核同质系相当于在ｍｔＤＮＡ水平上的近等基因系，所找到的多态就在所研究的目标基因附近，因此它是研究ｍｔＤＮＡ基因的良好体系。上述３个多态扩增结果在同核同质系（Ⅰ）的姐妹同核同质系（Ⅱ）中进行了验证，结果表明仅２个引物ＯＰＺ－１９、ＯＰＡＡ－１５在姐妹同核同质系（Ⅱ）的野生型正常可育株苏 ４７８（Ｆ）和其突变的不育株苏 ４７８－ｃｍｓ之间获得了与同核同质系（ Ⅰ）相同的多态性扩增产物ＯＰＺ－１９４２０、ＯＰＡＡ－１５６００。即这两个多态性产物在两套同核同质系中同时存在，因而这两个多态性产物与育性直接相关的可能性很大。更多还原

【Abstract】 Between wild fertile type (F) and its sterile mutant (cms), if their nucleus and cytoplasm are the same, this wild fertile type (F) and its sterile mutant (cms) are called isonucleus and isocytoplasmic lines. The maize mtDNAs of isonucleus and isocytoplasmic lines (Ⅰ), wild fertile type 478(F) and its sterile mutant 478-cms, were analyzed by RAPD. 94 primers were screened. 3 polymorphic products, OPZ-19420, OPAA-15600. and OPS-01400, were amplified between 478(F) and 478-cms. The results showed that mtDNAs in isonucleus and isocytoplasmic lines were more homologous than that in others. There is minor mtDNA difference between the fertile type (F) and its sterile type (cms) in a pair of isonucleus and isocytoplasmic lines. The polymorphism detected in isonucleus and isocytoplasmic lines may be more closely linked with the gene of fertility. Therefore, isonucleus and isocytoplasmic line is an excellent system in the study of CMS. Sister isonucleus and isocytoplasmic lines are consisted of 2 groups of isonucleus and isocytoplasmic lines in which their nucleus are not all the same but closely related, their cytoplasm are the same. Using sister isonucleus and isocytoplasmic lines is equal to determining the change of fertility by transferring one cytoplasm into the isonucleus. Isonucleus and isocytoplasmic lines (Ⅱ) are consisted of Su478(F) and Su478-cms. Isonucleus and isocytoplasmic lines (Ⅰ) and (Ⅱ) are sister isonucleus and isocytoplasmic lines. The polymorphic products, OPZ-19420. and OPAA-15600, can also be obtained in isonucleus and isocytoplasmic lines (Ⅱ). The 2 polymorphic products OPZ-19420 and OPAA-15600 are existed in both of the isonucleus and isocytoplasmic lines. This showed that isonucleus and isocytoplasmic lines are practicable in the study of CMS, and that common polymorphism in isonucleus and isocytoplasmic lines may be related more directly to fertility.更多还原