【摘要】 利用现有的转抗白叶枯病基因Ｘａ２１的水稻材料，通过ＴＡＩＬ－ＰＣＲ技术扩增出携带Ｘａ２１基因的Ｔ－ＤＮＡ的侧翼序列。对２４个有效扩增片段的序列分析结果表明，其中１４个侧翼序列是水稻ＤＮＡ，９个含载体主干序列，１个是外源基因Ｘａ２１片段。１４个Ｔ－ＤＮＡ侧翼的水稻ＤＮＡ序列与直接转化法外源基因整合位点的基因组序列具有不同的特点。这些Ｔ－ＤＮＡ在水稻染色体上整合后其两端序列的特点类似于在转基因双子叶植物中观察到的现象。在含主干序列的侧翼序列（３７．５％，９ ／ ２４）中，载体主干序列是以不同的类型出现的。更多还原

【Abstract】 Rice transformation mediated by Agrobacterium tumefaciens has technically matured to some extent, but the mechanics of T-DNA integration in transgenic rice remains largely unknown. Using thermal asymmetric interlaced PCR (TAILPCR), we analyzed the flanking sequences of T-DNAs in transgenic rice plants, in which the resistance gene for rice bacterial blight disease, Xa21, had been integrated stably. Sequence analysis of 24 fragments amplified by TAILPCR showed that of them 14 were rice genomic DNA, 9 contained vector backbone sequences, and one was a fragment of the exogenous gene Xa21. The characteristics of the 14 rice genomic DNA sequences at T-DNA integrated sites are significantly different from those of the reported rice genomic sequences into which exogenous genes were integrated through direct DNA transformation methods. T-DNA border sequences integrated in rice genome have similar features as those integrated in dicotyledonous genomes. In the backbonecontaining flanking sequences (37.5%, 9 ／ 24) vector backbones appeared in different types.更多还原