【摘要】 以４个普通小麦同核异质系——可育系Ｄ２－ＣＡ８０５７（ＢＣ１４）、Ｄ２型不育系ｍｓＤ２ －ＣＡ８０５７（ＢＣ１１）、 ＹＡ型不育系 ｍｓＡ－ＣＡ８０５７（ＢＣ１２）、 ＣＡ８０５７（核亲本）为材料，采用 ＰＡＧＥ 方法比较了４个系灌浆期种子胚乳和“花粉双核期”花药的过氧化物酶同工酶，采用梯度 ＳＤＳ－ＰＡＧＥ方法比较了４个系不同发育时期（干种子胚乳、越冬前和越冬后苗期叶片、“花粉 双核期”旗叶、“花粉双核期”花药）可溶性蛋白质的组份，发现可育系之间差异较小，不育系 与可育系之间、Ｄ２型不育系与 ＹＡ型不育系之间存在显著差异。表明细胞质（不育）基因的 表达具有时空性和特异性，这些差异（表达）带作为细胞质（不育）基因对核基因的特异调控 表达产物，可以作为 ４个系的生化标记，同时也表明 ｍｓＤ２－ＣＡ８０５７和 ｍｓＡ－ＣＡ８０５７是 ２个不 同的新不育类型。更多还原

【Abstract】 The peroxidase isozyme and the soluble peoteins of four isonucleusalloplasmic lines of common wheat-D2-CA8057 (fertile, BC14), CMS line msD2CA8057(BCll), CMS line msA-CA8057(BC12), CA8057 (nucleus donor) in different developmental stages were studies by polyacryldride gel electrophoresis (PAGE) and gradient SDS-PAGE respectively. The Peroxidase isozyme of bionucleus-stage anther, milk-stage endosperm and the soluble proteins of bionucleus-stage anther, leaf (the beginning and ending overwinter), dry-seed endosperm among fertoile line, msD2 CA8057 and msA-CA8057 were evidently different (but few differences between CA8057 and D2 -CA8057). These differences resulted from the Pedally-regulated expression of (male-sterile) cytoplasmic genes to nuclear genes and could be used to identify the four isonucleus-alloplasmic lines. These results showed that msD2-CA8057 and msA-CA8057 were two different kinds of new CMS pattern.更多还原