【摘要】 [目的 ]观察异尖线虫幼虫在大鼠体内移行径路 ,并进行病理学研究 .[方法 ]禁食 12h后 ,每只大鼠经口感染 10条虫体 ,感染后分别剖检 1,2只 ,后记录虫体状态、移行部位 ,病灶组织用 10 0 g/L福尔马林固定 ,苏木素和伊红染色 .[结果 ]异尖线虫幼虫在两组大鼠体内移行过程很类似 ,感染 1～ 5h虫体主要钻入胃壁 ,随食糜达肠腔 ,6h后开始侵入肠壁 ,继之进入腹腔 ,4 8h后侵入数减少 .两组大鼠末梢血象在感染 1d后嗜酸性粒细胞数稍增高 ,而占白细胞总数的百分比稍下降 ,7d后嗜酸性粒细胞数及百分比明显升高 .初次感染 2h后虫体周围出现少量嗜酸性粒细胞浸润 ,17h后浸润显著 ,在虫体头及食道周围出现放射状均质性嗜伊红物 ,72h后形成局灶性小脓肿 ,虫体脱皮 ,中肠细胞开始变性 .再感染 7h后虫体周围嗜酸性粒细胞浸润明显 ,虫体头及食道周围出现放射状均质性嗜伊红物 ,4 8h后形成蜂窝织炎 ,虫体开始脱皮及中肠细胞变性 .[结论 ]再感染组较初感染组宿主炎症反应剧烈 ,时间早 .更多还原

【Abstract】 OBJECTIVE? To observe the migration course of anisakis larvae and studied the pathologic changes in rats.?METHODS? After fasting for 12?h, each rat was infected with 10 worm by oral. 1 or 2 of infected rats were examined by autopsy in different time respectively, and the worm status and migration position was recorded. The tissues were fixed in 10% of formalin and done HE stain.?RESULTS? The migration course of anisakis larvae in two groups of Wistar rats were similar. The larvae mainly penetrated into the stomach wall and entered the intestinal cavity at 1～5 hours after infection, and began to penetrate into intestinal wall at 6?h, then entered abdominal cavity, the penetrated number reduced after 48?h. The number and the percentage of peripheral eosinophil were nearly normal or lower than normal. While at 7th day, the number and percentage were all arised. In non sensitized group, eosinophils began to gather around larvae at 2h after infection, and obvious at 17h. Radial homogeneous eosinophilic substance (RHES) found around the head and oesophagus of the larvae, localized abscess formed, larvae molted and intestinal cells began to degenerate at 72h. In the sensitized group, infiltration of eosinophils was obvious at 7h, phlegmon formed, larvae began to molt and intestinal cells of the larvae began to degenerate at 48h. It shows that the inflammatory reaction of the sensitized group was severer and earlier than that of non sensitized group.更多还原