【摘要】 目的　挤压大鼠颈总动脉 ,造成类似血管成形术后再狭窄的血管内膜增生病理模型。方法　暴露大鼠颈总动脉 ,在其上下各置一块 13mm× 5mm× 1mm钢片 ,以止血钳钳夹钢片 2 5min ,术后 2h、14d进行形态学观察。结果　术后2h ,扫描电镜可见血管内皮细胞变形、脱落 ,附有大量血小板。光镜可见血管壁有炎症细胞浸润 ,管腔内有血栓形成。术后 14d ,免疫组化血管壁PCNA表达强阳性 ,内膜面积占有率、中膜 +内膜面积占有率明显增加 ,管腔面积占有率明显缩小。类肝素、阿司匹林药物对PCNA表达及内膜增生有明显抑制作用。结论　挤压大鼠颈总动脉 ,可引起类似血管成形术后再狭窄的内膜增生病变更多还原

【Abstract】 AIM To establish a model of vascular intima hyperplasia for study the restenosis after angioplasty. METHODS Carotid artery of rat was exposed under general anesthesia. Two pieces of steel slides(13 mm×5 mm×1 mm) were set on and under the carotid artery separately. Then the slides were squeezed by two surgical forceps paralleling to the artery for 25 min. Histomorphological study was performed at the second hour or on the fourteenth day after operation. RESULTS At the second hour after operation, the loss of endothelial integrity and platelet deposition were seen under the scanning electromicroscopy. Under the light microscopy, there were infiltration of inflammatory cells in vessel wall andthrombus formation in the vessel cavity. At the fourteenth day after operation, PCNA positive cells in vessel wall of squeezed artery were significantly higher, the ratios of intima, medium and intima areas of squeezed artery were significantly increased and the ratio of cavity area of squeezed artery was significantly decreased compared with those of uninjured artery.The vascular intima hyperplasia and PCNA positive cells in squeezed arterial wall were inhibited by oral administration of heparinoid and aspirin. CONCLUSION With squeezing carotid artery in rats the vascular intima hyperplasia as restenosis after angioplasty was established.更多还原