【摘要】 利用刺囊毛霉(Mucor spinosus)3.3450的培养液对天麻素进行生物转化,得到其酶水解产物甙元对羟基苯甲醇,转化率接近100%。通过无细胞抽提液对底物转化的研究,证明催化该反应的酶为组成酶、胞外酶。更多还原

【Abstract】 Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of its 1H, 13C NMR and EI-MS spectral data. It could be inferred that the enzyme responsible for the biotransforma-tion reaction was a kind of extracellular and constitutive enzyme since the transformation reaction of the substrate could be carried out in cell free extracts of the fermentation broth of the Mucor spinosus.更多还原