【摘要】 目的　了解血吸虫病洲岛型流行区居民血清抗 AWA、重组 PMY和 Teg Ag的抗体亚类水平及其与居民再感染的关系。方法　 137例行 5 0 mg/ kg吡喹酮顿服。 7周后粪检 ,阳性者再次治疗 ,4周后经再次粪检证实为阴性。经 1个感染季节后再粪检并采血 ,用 EL ISA法检测抗 3种抗原的抗体亚类水平。结果　观察对象治前感染率 2 8.5 % ,治后再感染率 6 .6 % ;抗 AWA的 Ig G4和 Ig A峰值出现在 2 0～ 2 9岁 ,Ig E的峰值在 40～ 49岁 ,Ig G2 则在 2 0～ 2 9岁和 40～ 49岁 ,抗 AWA的 Ig E与 Ig G4的水平呈正相关 ;男性抗 AWA和 PMY的抗体亚类水平显著高于女性 ;感染状态不同的 4组中 ,再感染组抗 AWA的特异性总 Ig G和 Ig G4较其他 3组高。结论　特异性抗体水平与年龄之间没有明显关系 ;抗 AWA的 Ig G4可能在易感个体内起着一种封闭性抗体的作用 ;抗 AWA的 Ig E反应伴随着 Ig G4的反应。更多还原

【Abstract】 Objectives ”BZ]To observe levels of specific antibody isotype to adult worm antigen (AWA)of Schistosoma japonicum ”BZ]and recombinant paramyosin(PMY)and 22 kDa tegumental membrane associated antigen(TegAg),in the sera from residents in an area moderately endemic for schistosomiasis japonica, and to investigate the relationships between these levels and residents’ age, sex and status of infection and reinfection. Methods ”BZ]One hundred and thirty seven individuals in study area who have higher previous exposure levels were selected as the cohort subjects. These subjects were then treated with a single curative oral dose of praziquantel (50 mg/kg) in early of March 1996. Seven weeks later, a stool re exam by Kato Katz method was undertaken for these cohort subjects. The individuals who still were stool positive were re treated. Four weeks after re treatment, another stool exam was carried out for the re treated subjects, all re treated subjects were ensured that they were stool negative. An infective season later, all cohort subjects received a stool re exam, and serum samples were collected for the detemination of specific antibody isotypes. The levels of antibody isotypes were detected by quantitative ELISA method. Results ”BZ]The infection rate in cohort subjects pretreatment was 28 5%. Through one infective season after treatment, the reinfection rate was 6 6%. The specific IgG 4 and IgA antibody to AWA peaked in 20～29 years old age group, specific IgE peaked in 40～49 years old age group and specific IgG 2 antibody peaked in 20～29 and 40～49 years old age groups. IgE antibody to AWA correlated positively with specific IgG 4 levels. Levels of antibody isotypes to AWA or PMY in male were significantly higher than those in female. Levels of total IgG and IgG 4 to AWA in reinfected group were higher than those in groups with other different status of infection. Conclusions ”BZ]There was no notable relationship betweenlevels of specific antibody isotypes and age. IgG 4 antibody to AWA might act as a block antibody in the susceptible individuals and was accompanied by specific IgE response. Key Words To observe levels of specific antibody isotype to adult worm antigen (AWA)of Schistosoma japonicum ”BZ]and recombinant paramyosin(PMY)and 22 kDa tegumental membrane associated antigen(TegAg),in the sera from residents in an area moderately endemic for schistosomiasis japonica, and to investigate the relationships between these levels and residents’ age, sex and status of infection and reinfection. Methods ”BZ]One hundred and thirty seven individuals in study area who have higher previous exposure levels were selected as the cohort subjects. These subjects were then treated with a single curative oral dose of praziquantel (50 mg/kg) in early of March 1996. Seven weeks later, a stool re exam by Kato Katz method was undertaken for these cohort subjects. The individuals who still were stool positive were re treated. Four weeks after re treatment, another stool exam was carried out for the re treated subjects, all re treated subjects were ensured that they were stool negative. An infective season later, all cohort subjects received a stool re exam, and serum samples were collected for the detemination of specific antibody isotypes. The levels of antibody isotypes were detected by quantitative ELISA method. Results ”BZ]The infection rate in cohort subjects pretreatment was 28 5%. Through one infective season after treatment, the reinfection rate was 6 6%. The specific IgG 4 and IgA antibody to AWA peaked in 20～29 years old age group, specific IgE peaked in 40～49 years old age group and specific IgG 2 antibody peaked in 20～29 and 40～49 years old age groups. IgE antibody to AWA correlated positively with specific IgG 4 levels. Levels of antibody isotypes to AWA or PMY in male were significantly higher than those in female. Levels of total IgG and IgG 4 to AWA in reinfected group were higher than those in groups with other different status of infection. Conclusions ”BZ更多还原