【摘要】 用西葫芦作为罗汉果花叶病毒 (WMV 2 Luo)的繁殖寄主 ,通过差速离心和PEG二次沉淀法得到WMV 2 Luo的提纯液 ,用该病毒提纯液免疫日本大白兔 ,获得了WMV 2 Luo兔抗血清。经ELISA测定 ,其效价为 1/5 12 0 ,以 0 1mol/L磷酸盐缓冲液 (pH7 8,含 1 5 %NaDIECA)作为样品制备缓冲液 ,用间接ELISA法检测WMV 2 Luo的灵敏度为 1/32 0 0。用此方法检测罗汉果组培苗带毒情况和田间病株均获得成功。更多还原

【Abstract】 Luohanguo mosaic virus (WMV-2-Luo) were purified from Cucurbit pepo infected by WMV-2-Luo,and concentrated by one cycle of differential centrifugation and PEG double precipitation.An antiserum against WMV-2-Luo with a virus-specific titre of 1/5120 by ELISA was obtained by immunizing a Janpanese rabbit using the purified virus preparation.With a proper phosphate buffer solution,i.e.0.1 mol/L phosphate buffer(pH7.8,including 1.5%Sodium diethyldityiocarbamate)for preparing the crude plant sap to be detected,it was possible to increase the sensitivity of DAC-ELISA to a dilution of plant sap at a rate of 1/3200.It is successfully to detect WMV-2-Luo of the samples from tissue cultural planted and field.更多还原