【摘要】 目的 :研究唾液α -淀粉酶与致龋菌血链球菌粘附的关系 ,为龋病的病因学研究和预防提供理论依据。方法 :采用不同浓度α 淀粉酶及全唾液作为实验性获得性膜成分 ,观察经放射性同位素3H -胸腺嘧啶核苷酸标记了的血链球菌对羟基磷灰石的粘附情况。结果 :α 淀粉酶组与对照组相比除了 0 6 5mg/mlα -淀粉酶获膜对血链球菌的粘附作用无显著性外 ,2 0mg/ml、4 0mg/ml浓度的α -淀粉酶获膜能明显促进血链球菌对羟基磷灰石的粘附 (P <0 0 5 ) ,而且α 淀粉酶对血链球菌的促粘附作用随浓度增加而增加 (P <0 0 5 )。α 淀粉酶组与无龋正常人全唾液获膜组对血链球菌粘附程度的影响无区别 (P >0 0 5 )。结论 :α -淀粉酶促进血链球菌对羟基磷灰石的附着 ,然而当α 淀粉酶含量增加到一定浓度时 ,其对羟基磷灰石的粘附作用与全唾液组相等。更多还原

【Abstract】 Objective:to search the relationship between the α-amylase and the adherence of S.sauguis to hydroxyapatite.Methods:to observe the adherence of S.sanguis labelled with 3 H-TdR on hydroxyapatite covered separately by α-amylase in varying concentrations and whole saliva as experimental pellicles.Results:α-amylase significantly promoted the adherence of S.sanyuis to hyolroxyapatite,and the adherence was on the increase by increasing concentration.The effects of α-amylase and whole saliva from caries-free adults on the adherence of S.sanguis to hydroxyapatite were the same.Conclusion:α-amylase can promote and increase the adherence of S.sauguis to hydroxyapatite,until maximal adherence plateau concentration is reached.更多还原