【摘要】 本研究在对自然发病的中蜂欧洲幼虫腐臭病 (EFB)的病原蜂房蜜球菌进行分离鉴定的基础上 ,着重对蜂房蜜蜂球菌的敏感药物进行了筛选研究。研究分为三个层次 ,第一层 :临床常规用于蜂病防治的抗生素的研究 ;第二层 :中草药有效成份提取物的研究 ;第三层 :应用均匀设计原理进行中草药配方的研究。通过以上研究 ,拟对EFB防治药物的更新换代和“绿色蜂药”的使用打下基础。更多还原

【Abstract】 The honey bee EFB pathogen was isolated from the dead Apis cerana cerana larvae naturaly infected with EFB.According to the cultural,morphological,physiological and biochemical,serological characteristics;and the results of molecular biological test and infectivety test the pathogen was identified as Melissococcus pluton.The sensitivity test divided into three steps.1 studing the antibiotics commonly used in honey bee diseases.The results showed that the pathogen was very sensitive to Erythromycin,Josamycin,Midecamycin,Cefalexin but was resistant to Sulfamethoxazole timethoprim and Norfloxacin.2 Studying the MIC and MBC of effective composition extraction from some traditional Chinese medicine,the results showed that Kandofort MIC 1;640,the medicine content was 3 125 μl/ml,MBC 1∶20,50 μl/ml.Another medicine′s MIC 1∶1280,the medicine content was 781 2 μg/ml,MBC 1∶320,3125 μg/ml.3 Using Uniform Design method to manage test data,we used 8 kinds of traditional Chinese medicine and made up two effective prescriptions for EFB.更多还原