【摘要】 用鸡痘病毒载体表达马立克氏病病毒 (MDV)糖蛋白B(gB)基因构建成重组鸡痘病毒 (rF PV)。以MDVGA或Md5和RBIB混合攻毒 ,在不同品种的鸡中评价rFPV -gB/R单价苗和与火鸡疱疹病毒 (HVT)组成的二价苗的免疫保护效力。试验结果表明 ,MDV疫苗和FPV母源抗体阴性的SPF鸡和母源抗体阳性的商品鸡中 ,rFDV - gB/R均能提供免疫保护作用 ,且其中一种商品蛋鸡中rFPV - gB/R与HVT液氮苗或HVT冻干苗结合使用 ,均有显著的免疫协同保护作用。免疫学研究指出 ,rFPV -gB/R与常规疫苗一样 ,可显著地降低疫苗免疫攻毒鸡的病毒血症和羽囊排毒。更多还原

【Abstract】 Recombinant fowlpox Virus (rFPV) was constructed to express glycoprotein B (gB) gene from CVI988/Rispens strain of Marek’s disease virus (MDV).The rFPV-gB/R alone and in combination with herpesvirus of turkey (HVT) preparations were evaluated for their protective efficacy against challenge with different MDV strains in different chickens.The rFPV-gB/R alone induced protection comparable to that by HVT vaccines in both AbSPF chickens and Ab+ production chickens.Significant protective synergism was observed in one of the two commercial production chickens when rFPV-gB/R was combined with HVT of either cell associated or cell free preparations.Immunogenesis studies showed that rFPV-gB/R,just like conventional vaccines,significantly reduced the level of viremia and feather follicle shedding with virus in vaccinated chickens.更多还原