Objective To determine whether human nonpigmented ciliary epithelial cells can physiologically synthesize artial natriuretic peptide (ANP) on the transcriptional level. Methods Human nonpigmented ciliary epithelial cells were isolated and cultured in vitro. Total RNA was extracted from those cells when primary culture became confluent. ANP message RNA(mRNA) was identified by reverse transcription-polymerase chain reaction (RT-PCR). Results ANP mRNA existed in human nonpigmented ciliary epithelial ce...
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