【摘要】 [目的]观察外周血T淋巴细胞脱氧核糖核酸核蛋白体(rDNA)转录活性对消化系统恶性肿瘤的诊断有无临床价值 ,探讨其变化规律。[方法]选择全部经病理证实的消化系恶性肿瘤患者80例 ,以122例非恶性肿瘤患者作对照 ,采用微量全血培养72小时后制片 ,计算机分析细胞核的银染核仁积分面积 /细胞核积分面积(I.S) ,银染核仁积分光密度 /细胞核积分光密度(I.O.D)。[结果]非肿瘤组 :I.S :(9.01±2.34) % ,I.O.D:(8.05±2.03) % ;肿瘤组 :I.S:(5.98±1.82) % ,I.O.D :(4.56±1.95) % ,差异显著(P<0.01)。非肿瘤组淋巴细胞核仁银染颗粒大 ,数目多 ,染色深 ,大部分超过5个 /核。而肿瘤组颗粒较小染色浅 ,在1～3个 /核 ,差异较大。[结论]本研究结果表明消化系恶性肿瘤组患者外周血T淋巴细胞核仁组成区相关嗜银蛋白 (AgNOR)的嗜银强度明显低于非肿瘤患者 ,说明消化系恶性肿瘤rDNA转录活性明显低于非肿瘤患者。因此 ,检测外周血T淋巴细胞rDNA转录活性的变化可作为消化系统恶性肿瘤辅助诊断的一项指标更多还原

【Abstract】 To investigate the transcription activity of ribosome deoxyribonucleic acid (rDNA) in peripheral blood T lymphocyte for the diagnosis of digestive system malignant tumor.Eighty cases of patients with malignant tumors of digestive system proved pathologically were investigated in comparison to 122 cases of patients with non_malignant tumors.The slidies were made after minimum blood cells were cultured for 72 hours.rDNA transcription activity was detected by cell analysis of nucleolar organizer region associated argyrophil proteins (AgNOR).I.S and I.O.D were analyzed by use of computer.Non malignant tumor group:I.S(9.01±2.34)%,I.O.D( 8.05±2.03)%;malignant tumor group:I.S (5.98±1.82)%,I.O.D( 4.56±1.95)%;there were significant differences between two groups ( P<0.01 ).There were smaller granula (1～3/ nucleus ) and hypochromatism in malignant tumor group and larger granula (over 5 / nucleus ) and hyperchromatism in non_malignant tumor group.[Conclusion]rDNA transcription activity is markedly lower in malignant tumor than that in non_malignant tumor, and testing changes of rDNA transcription activity may serve as a marker in the auxiliary diagnosis of malignant tumors of digestive system.更多还原