【摘要】 目的 :观察 tirofiban对犬髂动脉球囊损伤后血浆可溶性粘附分子 CD6 2 p水平及全血白细胞计数的影响。方法 :2 4只健康成年犬随机分为治疗组 (tirofiban 30 0μg/ kg静推 ,后以 0 .3μg· kg- 1 · min- 1 静滴 6 h)和对照组 (9g/ L 盐水静滴 ) ,每组 12只。行单侧髂动脉球囊损伤 ,术前、术后即刻及 2 ,4,6 h静脉取血行全血白细胞计数 ,EL ISA法测定血浆 CD6 2 p含量 (光密度 )。结果 :对照组球囊成形术后血浆 CD6 2 p含量增加 ,2 h后开始下降 ;而治疗组 CD6 2 p术后立即下降 ,4h后恢复至基线。全血白细胞在对照组术后逐渐增加 ;而治疗组用药后则出现一过性白细胞受抑。结论 :tirofiban除了已知的抑制 GP b/ a受体和血小板聚集外 ,还能抑制球囊成形术后血小板活化及全血白细胞。可能通过抑制 CD6 2 p(P- selectin)、抑制白细胞 -血小板间相互作用改善经皮冠状动脉介入治疗后的临床结果。更多还原

【Abstract】 AIM:To observe the effects of GPⅡb/Ⅲa antagonist tirofoban on level of plasma soluble adhestion molecule CD62p and whole blood leukocyte counts in canine model of iliac artery injury by balloon angioplasly. METHODS:24 health adult dogs were randomized into the treated group (tirofiban 300 μg/kg bolus+0.3 μg·kg -1 ·min -1 continuous venous injection for 6 h) and the control group (treated with saline). Iliac artery injury by balloon angioplasty was performed 15 min after the drug injection. Blood samples were drawn through femoral vein catheter. Whole blood leukocyte counts were measured mechanically and plasma CD62p concentration(A) was measured by ELISA 15 min before and after procedure every 2 h for 6 h. RESULTS:CD62p level was increased after angioplasty and began to decrease 2 h after the procedure in the control group,while reduced immediately and then increased to baseline 4 h after angioplasty in the treated group. Whole blood leukocyte counts were increased after the procedure in the control group,whereas a transient decrease after the administration of tirofiban was observed in the treated group. CONCLUSION:In addition to its known effect on platelet aggregation by block GPⅡb/Ⅲa receptor,tirofiban can inhibit platelet activation after angioplasty and decrease leukocyte counts transiently. The outcome improvement on percutaneous coronary intervention by tirofiban may be acquired through inhibition of CD62p(P selectin) and interaction of leukocyte and platelet.更多还原