【摘要】 用 RAPD技术分析我国常见家鸭品种 (系 )金定鸭 ( I、II、III系 )、绍兴鸭、巢湖鸭、大余鸭、莆田黑鸭 ( I、II系 )、连城白鸭、北京鸭 ,及引进品种番鸭和野鸭斑嘴鸭、绿头鸭基因组 DNA的多态性 .2 4个引物中 ,有 19个共扩增出 2 83条带 ,分子量约在 4 0 0～ 2 0 0 0 bp之间 ,13个样品共有的条带数只有 2 7条 .根据扩增得到的 RAPD图谱计算了不同样品间的遗传距离 ( D)和遗传相似系数 .绿头鸭、斑嘴鸭与家鸭品种 (系 )间的遗传距离分别在 0 .2 2 4 0～ 0 .30 92和 0 .2 880～ 0 .3735之间 ,方差分析表明两组数据不存在显著差异 ( p>0 .0 5) .并依据 D值由 UPGMA聚类分析软件绘制了它们的分子进化树 .更多还原

【Abstract】 Random amplified polymorphic DNA (RAPD) was used to estimate the genetic diversity among domestic ducks and two species of wild duck: Twenty four 10 bp single primers of arbitrary sequence were used to amplify mixed genomic DNA. Most of the primers got clear fingerprints except five. 283 amplified bands were scored and only 27 of them shared by all of the samples. The genetic distance ( D ) between domestic ducks and Anas Platyrhynchos was 0.224 0~0.309 2, and Anas Poecilorhyncha’s was 0.288 0~0.373 5. The variance between them was not significant ( p >0.05). The D value was used to construct a dendrogram by unweighted pair group method with arithmetical averages.更多还原