【摘要】 研制出一种智能化毛细管电色谱仪 ,介绍了该仪器的工作原理、构成和特点。设计了一种包括具有极小死体积的进样器、恒压分离系统和适于梯度洗脱的分流器的流路结构 ,可有效地解决CEC现存的主要问题。仪器的自动控制系统基于Intel80C196单片机 ,包括A/D ,D/A转换和串口通信等模块 ,控制系统通过串行口与PC机通信 ,读取用户设置的参数和程序 ,完成对高压电源和泵的控制。在同一台仪器上可实现电压驱动电色谱、压力驱动电色谱、微柱液相色谱和毛细管电泳等多种分离模式。测试了该仪器的分离重现性和自动控制的可靠性 ,获得满意结果。该仪器采用梯度加压毛细管电色谱已成功地用于 10条DNA片段分离更多还原

【Abstract】 An intelligent capillary electrochromatography instrument was developed and its working principle,construction and characteristics are described.A zero dead volume microinjector,a constant pressure separation system and a flow splitter for gradient elution are designed,which are effective for solving the existing main problems of CEC.The automation system of the instrument is based on Intel 80C196 micro chip system consisting of A/D,D/A serial communication parts,its controller gets experiment parameters through serial from PC and actuates the pump and the high voltage source by D/A and serial communication.Different separation modes such as CEC,p CEC,μ HPLC,and CE can be achieved on only the one.The reproducibility of separation and the reliability of automatic control were tested with satisfactory results.The instrument has been successfully applied to a separation of ten DNA fragments by gradient pressurized capillary electrochromatography.更多还原