【摘要】 目的 :通过病人、宿主和媒介标本的病原学分析 ,阐明河北省恙虫病疫源地。方法 :采集疫源地内病人血标本、鼠和恙螨标本 ,采用昆明种小白鼠传代分离法 ,脏器涂片 Giemsa染色和脏器速冻切片用免疫荧光法镜检恙虫病立克次体 Rickettsia tsutsugamushi,Rt) ,用 PCR方法检测 Rt核酸 ,用免疫荧光法检测特异性血清抗体和血清学分型。结果 :捕捉鼠肝脾肿大率 6 7.7% ,血清抗体阳性率 2 2 .0 %。 96 .9%的捕捉鼠肝脾标本、 88.9%的病人血标本及小板纤恙螨标本引起实验鼠发病和死亡 ,实验鼠发病症状和体征符合恙虫病的感染 ,实验鼠脏器涂片和速冻切片均镜检到 Rt,PCR方法检测到 Rt核酸 ,病人血清分型为 Rt Gilliam型。结论 :从病原学进一步证实了河北省恙虫病疫源地的存在更多还原

【Abstract】 Objective:To clarify the epidemic focus of tsutsugamushi disease by etiological analysis of samples for the patients sera,animal hosts and vectors.Methods:Samples were collected for the patients sera,mice and trombiculid mites.Rickettsia tsutsugamushi(Rt) were isolated by inoculation small white mouse with Kunming species.Giemsa decoration for liver or spleen smear,and immunofluorescent method for freezing cut section of liver and spleen,were used to test Rt.Nucleic acid of Rt was examined by PCR.Results:The rate of hepatomegaly and/or splenomegaly,prevalance of Rt antibody for mice caught in the epidemic focus,were 67 7% and 22 0%,respectively.96 9% of liver and spleen specimens for mice caught,88 9% of blood specimens for the patients,and Leptotrombidium scutellare caused illness or death of experimental mice.The symptoms of attacking mouse accord with Rt infection.Rt nucleic acid were all tested out in the smears,freezing cut sections and tissue specimens.The serotype was Gilliam of Rt.Conclusion:The epidemic focus of tsutsugamushi diaease in Hebei province was demonstrated in etiology in further.更多还原