【摘要】 目的　研究肿瘤浸润性淋巴细胞 (TIL)对肺癌细胞株 (A5 49)的致凋亡作用及其作用机理 ,为临床应用TIL治疗肺癌提供依据 .方法　从肺癌病人胸水中用淋巴细胞分离液分离提取出TIL细胞 ,在含 10 0 0U/mlIL - 2的完全培养基中培养 2 0天后 ,将TIL细胞与靶细胞 (肺癌细胞株 )共同培养 2 4小时 ,将培养细胞制成细胞涂片 ,经福尔马林固定 ,用原位末端脱氧核苷酸转移酶 (TdT)介导的脱氧三磷酸嘧啶 (duTP)末端标记法 (TUNEL)原位的方法进行肺癌凋亡细胞的标记 .结果　肺癌肿瘤细胞凋亡指数 ( % ) :经TIL作用组的凋亡指数为 8.5 6± 0 .84,对照组凋亡指数为2 .10± 0 .86 ,两组之间差别有非常显著性 (p <0 .0 1) .结论　TIL细胞诱导肺癌细胞的凋亡可能是TIL细胞对肺癌细胞杀伤作用的机理之一更多还原

【Abstract】 Objective The function of induced apoptosis of tumor infiltrating lymphocytes (TIL) to lung carcinoma cell line (A549) was studied in this paper.The basic data of study was provided for the clinical application of TIL’s treatment to lung carcinoma. Methods The TIL cells were separated from pleural effusion of lung carcinoma patients by Ficoll-Hypaque ( 1.077±0.001 ) After cultured for 20 days in completed medium with 1000U/ml IL-2, TIL cells co-cultured with target cells (carcinoma cell line) for 24 hours. Then cultured cells were smeared on the slides, and fixed the slides in 10% formalin.Apoptotic carcinoma cells were labeled by TdT-mediate dUTP nick end labeling(TUNEL) method.Results Apoptotic index (%)of carcinoma cells was:8.56±0.84 in TIL experimental group, 2.10±0.86 in control group.There was different significance between the above two groups(p<0.01).Conclusions Apoptosis of lung carcinoma cells induced by TIL cells may be one of the mechanisms of TIL cells killed lung carcinoma cells.更多还原