【摘要】 目的 :用 17β 雌二醇 (E2 )与N 甲基 N 硝基 N 亚硝基胍 (MNNG)诱导ICR小鼠子宫内膜癌 ,研究子宫内膜癌变的诱发因素。方法 :选择 6周龄ICR雌性小鼠 90只 ,分为 4组。Ⅰ组 (空白对照组 ) ,小鼠 12只 ,常规喂养 ;Ⅱ组 (MNNG +E2 组 ) ,小鼠 2 6只 ,经阴道宫腔给予MNNG ,每周 1次 ,共 3次 ,在给予MNNG的同时喂E2 ;Ⅲ组 (MNNG组 ) :小鼠2 6只 ,仅给予MNNG ;Ⅳ组 (E2 组 ) :小鼠 2 6只 ,仅给予E2 。于实验后 3个月、6个月及 9个月各组取若干只小鼠 ,测定血清雌激素 (E)及孕酮 (P) ,并作子宫内膜病理组织学检查。结果 :实验 3个月时 ,Ⅳ组子宫内膜发生复杂型 (3 5 )或不典型增生性改变 (2 5 ) ;实验 6个月时 ,Ⅱ组、Ⅲ组、Ⅳ组均有部分发生复杂型或不典型增生过长及癌变 ,但 3组间差异无显著性 (P >0 .0 5 ) ;实验 9个月时Ⅱ组复杂型增生、不典型增生、内膜癌分别为 35 .7% (5 14)、2 8.6% (4 14)、35 .7% (5 14) ,Ⅳ组以上病变分别为 14.3% (2 14)、4 2 .9% (6 14)、4 2 .9%(6 14) ,Ⅱ组、Ⅳ组间相比差异无显著性 (P >0 .0 5 )。实验 6个月与 9个月时 ,喂E2 组E P均明显高于喂水组 (P <0 .0 1或P <0 .0 5 )。结论 :单独给予E2 或MNNG后 ,即能较好地诱发子宫内膜癌。E2 与MNNG联合给药时 ,效果并未较?更多还原

【Abstract】 Objective:To develop an animal model for endometrial neoplasms in ICR mice treated with 17beta-estradiol(E 2) and N-methy-N-nitro-N-nitrosoguanidine(MNNG). Methods:A total of 90 female ICR mice,6 weeks of age,were treated as follows:Group 1(12 mice) was fed with water.Group 2(26 mice) was given intravaginal instillation of MNNG solution once a week for three weeks and fed diet containing E 2.Group 3(26 mice) was given MNNG alone.Group 4(15 mice) was given E 2 diet alone.Small numbers of animals in four groups were killed at 3,6 and 9 experimental months for uterine histological and endocrinological examination.Results:At 3 experimental months,endometrial complex hyperplasia(3/5) and atypical hyperplasia(2/5) were found in group 4.At 6 experimental months,there were some complex hyperplasia,atypical hyperplasia and cancer in group 2,3,4,but with no significant difference within the three groups.At 9 experimental months,the incidences of complex hyperplasia of endometrium,atypical hyperplasia and endometrial cancer were 35.7%(5/14),28.6%(4/14),35.7%(5/14) respectively in group 2 and 14.3%(2/14),42.9%(6/14),42.9%(6/14) respectively in group 4.The E/P ratio in mice fed with E 2 was significantly higher than that with water at 6 experimental months (P<0.01)and 9 experimental months(P<0.05).Conclusions:There are many complex or atypical hyperplasias of endometrium and endometrial cancers in mice treated with E 2 or MNNG singly.Combined therapy is not better than single therapy.Increased E/P ratio might act as a promoter of development of endometrial proliferative lesions.更多还原