【摘要】 利用病菌接种鉴定、抗性遗传分析、基因推导和 STS方法 ,对抗白粉病小麦新种质 YW2 4 3进行鉴定。YW2 4 3高抗我国白粉病菌毒性级别在 7级以下、毒性频率占 90 %以上的生理小种 ,抗性有效 ,可在我国大部分麦区使用。与感病品种中国春、京 771测交的 F2 抗感分离符合 3∶ 1比例 ,证明YW2 4 3对白粉病的抗性由 1对显性基因控制。基因推导、抗性遗传分析和 STS- PCR产物的 RFL P带型分析结果表明 ,其抗性基因为 Pm4 b。STS- PCR产物的 RFLP带型分析发现限制性内切酶 H ind 酶切的 Pm4 a和 Pm4 b扩增产物有多态性 ,根据带型差异可鉴别这两个等位基因 ,该结果尚未见到有关报道更多还原

【Abstract】 The wheat line YW243 was evaluated using resistance test, gene to ge ne and genetic analysis and STS. The results indicated the line with an efficien t resistance was able to resistant to 1～7 group races which virulence frequency is above 90% in China. Test crossing were carried out using susceptible wheat cultivars Chinese Spring (CS) and Jing 771. It showed a pair dominance gene con t rolled YW243 with powdery mildew resistance. The gene to gene, genetic analysis and RFLP analysis of STS PCR band confirmed that the resistance gene of YW243 may be Pm4b . RFLP analysis of STS PCR band can used to differentiate Pm4a and Pm4b.更多还原