【摘要】 3组雏鸡分别于 1日龄和 1 0日龄采用滴鼻 +滴鼻、滴鼻 +肌注和肌注 +肌注程序 2次接种烟曲霉菌丝灭活苗或福氏完全佐剂灭活苗。二免后第 1 1天采血检测各组免疫鸡血清抗体水平 ;第 1 2天采用相同剂量 ( 5× 1 0 9孢子 /只 )烟曲霉菌孢子攻毒感染 ,测定各组雏鸡免疫保护率、生长发育及肺脏与大脑烟曲霉菌分离率。结果表明 ,各免疫组雏鸡的血清抗体水平、免疫保护率、生长发育与霉菌分离率差异不明显 ,血清抗体OD值为 0 .53～ 0 .82 ,免疫保护率为 70 %～ 85% ,比对照鸡平均多增重 33.6 4～ 6 2 .92 g( 36日龄 ) ;存活雏鸡肺脏霉菌分离率 4 1 .2 %～ 57.1 % ,含量 2 .4× 1 0 4～ 4 .3× 1 0 5 孢子 /g,大脑霉菌分离率 2 3.5%～ 35.7% ,含量3.3× 1 0 3 ～ 2 .4× 1 0 4孢子 /g。表明滴鼻接种与肌注免疫一样可以引起血清抗体应答 ,最佳免疫程序为 1日龄和 1 0日龄分别滴鼻接种烟曲霉菌丝灭活苗和肌注烟曲霉菌丝福氏完全佐剂灭活苗更多还原

【Abstract】 Three groups of chicks were vaccinated two times at 1 and 10 days of age with the methods of intranose and intranose, intranose and intramuscle as well as intramuscle and intramuscle injection, respectively. Levels of antibody in the sera of vaccinated and nonvaccinated chicks were determined 11 days after the last vaccination.Challege exposures were conducted with 5×10 9 CFU spores of A. fumigatus per millilitre 12 days after the last vaccination to determine protective rates, body weight and recovery rates of moulds from lungs and brains in every group of chicks.The results showed that the differences of serum antibody levels, protective rates, body weights and recovery rates of moulds were not significant among the three groups of vaccinated chicks.The OD values of serum antibody were 0.53～0.82.Protective rates were 70%～80%.Body weights were 33.64～62.92 g higher than that of nonvaccinated control group.Recovery rates were 41.2%～57.1% and 23.5%～35.7% and numbers of moulds were 2.4×10 4～4.3×10 5 and 3.3×10 3～2.4×10 4 spores/g in the lungs and brains of survivor at the end of test, respectively.The results demonstrated vaccination through hose can induce antibody reaction in the sera as through muscle injection,the best vaccination procedure is inoculated intranosely and intramuscldly with killed A.fumigatus vaccine and freunds adjuvant vaccine at 1 and 10 days of age.更多还原