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金花茶叶酶法提取工艺条件的研究
Studying on Enzymatic Extraction Process Condition of Yellow Flower Camellia
【摘要】 用添加外源酶把金花茶叶中的有效成分提取出来 .其最佳提取条件为 :加水量为金花茶叶干样的 10 0倍 ,添加的木瓜蛋白酶的质量分数为 0 .4 % ,pH值为 5 .0~ 5 .5 ,温度为 5 0~ 5 5℃ ,恒温12 0min .在此条件下 ,金花茶叶水浸出率、可溶性糖和氨基酸含量分别比对照增加 39.4 7%、7.39%和 1.84 % ,粗蛋白氨基酸的总浸出率为 2 5 .5 %左右 ,金花茶叶中矿质元素Fe、Zn、Se、Mn、Co、Mo、Ge、V的浸出率都在 95 %以上 .
【Abstract】 This optimal extraction parameters and the extraction rate of nutrient components of yellow flower camellia (C.Chrysantha(Hu) Tuyuma) were studied by enzymatic hydrolysis. With a ratio of 1∶100 between yellow flower camellia and water, an addition of 0.4% exogenous papain, pH 5.0~5.5, most of nutrient components and microelements were extracted at 50~55 ℃ for 2 hours. Under the above conditions, there were 39.47% increase of extraction rate of soluble solid, 7.39% increase of total soluble sugar and 1.84% increase of the extraction rate of crude protein amino acid by enzymatic hydrolysis, compared with by pure water extraction. The hydrolytic rate of crude protein was up to 25.5% and the extraction rate of Fe?Zn?Cu? Se?Mn?Co?Mo?Ge?V was up to 95% respectively in the optimal conditions.
- 【文献出处】 无锡轻工大学学报 ,Journal of Wuxi University of Light Industry , 编辑部邮箱 ,2001年06期
- 【分类号】TS275.2
- 【被引频次】34
- 【下载频次】303