【摘要】 通过质粒转化实验 ,获得了较好的产hEGF重组菌 ,对重组菌发酵条件进行优化 ,获得最佳初始糖质量浓度为 5 g/L、蛋白胨 2 0g/L、酵母抽提物 10 g/L、(NH4 ) 2 HPO4 3 .5 g/L、Amp10 0mg/L;最佳接种时间为种子液生长到 5～ 6h ,即菌体OD值在 1.0～ 2 .0 ;诱导剂最佳添加时间为 8h ,即菌体OD值在 8.0左右 .通过流加发酵进行高密度培养 ,可使重组菌的hEGF的产率达10 2mg/L ,比优化前提高了近 30 % .更多还原

【Abstract】 Plasmid was transferred into E.coli JM101.The fermentation condition of recombination strain was studied .The optimized mediums were glucose 5 g/L,peptone 20 g/L,yeast extract 10 g/L,(NH 4) 2HPO 4 3.5 g/L and Amp 100 mg/L.The best incubating time was between 5～6 hour,and the cell concentration was 1.0～2.0 of the OD.The induced time was 8 hours after the fermentation,the OD was about 8.0.By fed batch fermentation,the production of hEGF had dramatically increased,reached 102 mg/L,30% higher than before.更多还原