【摘要】 对重组大肠杆菌进行培养条件的研究 ,得到细胞合成L DOPA最佳培养条件为 :L tyro sine 0 .2 %、KH2 PO40 .2 %、MgSO4·7H2 O 0 .1 %、FeSO4·7H2 O 0 .0 0 1 %、ZnSO40 .0 0 1 %、磷酸吡哆醛 0 .0 0 5%、牛肉膏 0 .5%、蛋白胨 2 %、DL Alanine 0 .1 %、温度 37℃、pH值 7.0、培养时间 1 6h、摇瓶转速 1 50r/min .更多还原

【Abstract】 Culture conditions for recombinant E.coli containing high tyrosine phenol lyase were studied. Optimum condition of enzyme formation was cells cultured at 37 ℃for 16hours in the medium containing 0.2% L tyrosine, 0.2%KH 2PO 4, 0.1% MgSO 4·7H 2O, 0.001% FeSO 4·7H 2O, 0.001% ZnSO 4·7H 2O, 0.005% pyridoxal phosphate, 0.5% meat extract, 2% polypeptone, and 0.1% DL Alanine. Initial pH and rotation rate were 7.0 and 150 r/min respectively.更多还原