【摘要】 通过三亲本杂交将含有花生根瘤菌吸氢基因的质粒pZ 55(Tcr)转入不吸氢的花生根瘤菌Ra 34等菌株 (Hup- ,Nif+,Apr)中 ,筛选到既具有吸氢又具有固氮能力的花生根瘤菌结合株Rz34 2。在自生和共生条件下 ,结合株均可表达高吸氢和高固氮活性。以结合株Rz34 2接种的花生植株叶片的干重比不接种的、接种受体株Ra34(Hup- )和接种对照菌株L8 3(Hup+,Nif+)的分别高 6 2 %、7 6%和 6 3% ;种子的含氮量分别高 8 9%、1 0 0 %和 6 0 % ;产量分别高 1 8 8%、1 0 5%和 1 0 7%。研究结果表明 ,以含吸氢基因的结合株接种花生能提高根瘤菌与花生的共生固氮效率 ,增加作物的产量。更多还原

【Abstract】 The hydrogen\|uptake genes were transferred into wild Rhizobium arachis Ra strains (Hup -,Nif +,Ap r)by triparental mating using pRK2013 as help plasmid.A transconjugant R.arachis Rz34\|2(Hup +,Nif +,Ap r,Tc r)which expressed high activities of hydrogenase and nitrogenase under free\|living and symbiotic state was screened.Peanut inoculation test with recipient R.arachis Ra34,transcojugant Rz34\|2 and control strain R.arachis L8\|3 (Hup +,Nif +)was carried out respectively.The results showed that,compare to treatment without inoculation\,inoculation with R.arachis Ra34 and R.arachis L8\|3,the dry weight of leaf inoculated with transconjuant Rz34\|2 increased 6.2%\,7.6% and 6.3% respectively;the N\|content of seed increased 8.8%\,10.0% and 6.0%;the output increased 18.8%\,10.5% and 10.7%.This suggested that legume plants inoculated with Rhizobium strains (Hup +)were more efficient to accumulate N and to increase its output.更多还原