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特异扩增BBTV基因组Ⅲ、Ⅳ、Ⅰ编码区部分序列及其在检测中的应用

SPECIFIC AMPLIFICATION OF THE CODING SEQUENCES OF BBTV Ⅲ,Ⅳ,Ⅰ AND THEIR APPLICATION IN BBTV DETECTION

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【作者】 孙德俊毛国杰孙卉腊平蔡文启

【Author】 Sun Dejun\ Mao Guojie\ Sun Hui\ La Ping\ Cai Wenqi (Institute of Microbiology, The Academy of Chinese Sciences, Beijing\ 100080,China)

【机构】 中国科学院微生物研究所!北京100080

【Abstract】 Banana bunchy top virus disease (BBTD) is a disastrous disease in bananas, and it is spreading in the world (including China) by the banana bunchy top virus(BBTV). At present, virus\|free plantlets are used to prevent BBTD in banana production, therefore, it is very important to establish a method to detect BBTV quickly, sensitively and specifically. ELISA is now popularly used to detect BBTV. The sensitivity of this method is not high enough, and needs specific antiserum, otherwise, pseudo\|positive results often occur. According to DNA coding sequences of component Ⅲ,Ⅳ and Ⅰ of BBTV isolates from Zhangzhou, China, three pairs of primers are designed to establish a PCR method to specifically amplify parts of coding sequences of the BBTV coat protein, movement protein and replicase\|association. This method is also applicable to detect BBTV of bananas or cultured banana seedlings in other regions.

【关键词】 BBTV-DNA-ⅢⅣ和ⅠPCR检测克隆
【Key words】 BBTV DNA ⅢⅣ and ⅠPCRDetecti
【基金】 中国科学院“九五”院重大项目!(KY951 A1 30 2 1 2 1 0 )&&
  • 【文献出处】 微生物学报 ,Acta Microbiologica Sinica , 编辑部邮箱 ,2001年01期
  • 【分类号】Q939.4
  • 【被引频次】1
  • 【下载频次】25
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