【摘要】 用基因重组技术将截短的HIV 1p2 4基因和gp41基因连接成嵌合基因 ,插入质粒 pGEX 4T3,构建成重组表达质粒pGEX F。将 pGEX F转化大肠杆菌BL2 1。经IPTG诱导表达 ,pGEX F在大肠杆菌BL2 1中获得了高效表达。融合蛋白P2 4 gp41经Glutathione Sepharose4B亲和层析纯化后 ,用间接ELISA和免疫印迹检测HIV抗体阳性血清和正常人血清 ,P2 4 gp41只与HIV抗体阳性血清反应 ,证明获得的融合蛋白P2 4 gp41有很强的抗原特异性和免疫反应性 ,具有较高的应用价值更多还原

【Abstract】 To prepare a multivalent antigen of HIV 1 for diagnostic reagent,a recombinant expression plasmid pGEX F was constructed by inserting a chimeric gene encoding a fusion protein p24 gp41 of HIV 1 into plasmid pGEX 4T3.The pGEX F was trasfered into E.coli(BL 21 )and expressed by IPTG induction.The recombinant fusion P24 gp41 was purified by Glutathione Sepharose 4B affinity chromatography and characterized with indirect ELISA and Western blot.The recombinant fusion protein P24 gp41 was highly expressed in E.coli(BL 21 )and the purified P24 gp41 only reacted with HIV antibody positive serum in above tests.The recombinant P24 gp41 has excellent antigenigcity and immunoreactivity in relation to prosperous application.更多还原