【摘要】 目的　建立一种简便的将人源 Ig G抗体转化成盐水活性抗体的方法。方法　用二硫苏糖醇 (DTT)等物质 ,制备成免疫球蛋白改性剂 (ICR) ,直接处理 Ig G性质 Rh抗血清 ,无须另外烷基化和透析。结果　用 ICR和传统DTT法同时处理 16份效价为 8～ 10 2 4的 Ig G性质 Rh抗体 (13份抗 - D、2份抗 - E、1份抗 - C) ,结果 ICR法均制备出盐水抗体 ,而传统法仅从 6份较高浓度 Ig G血清中制备出盐水抗体。将 ICR制备的盐水抗 - D用于供血者的 Rh血型检测 ,效果良好。结论　该方法简便、重复性好 ,受原料血清中 Ig G抗体浓度影响小 ,成品保存时间长更多还原

【Abstract】 Objective\ To develop a simple method to convert human IgG anti-sera into active saline antibodies. Methods\ Immunoglobulin converting reagent(ICR) prepared from dithiothreitol(DTT) etc, was used to directly process IgG Rh anti-sera, without additional employment of alkylation and dialysis.Results\ 16 IgG Rh anti-sera of titre 8～1024(13 anti-D,2anti-E and 1 anti-C) were treated with parallel ICR and traditional DTT method. Saline active antibodies were produced from all IgG sera by using ICR method, while in the case of traditional DTT method, the saline solution antibodies produced from only 6 high titre IgG sera. The result was satisfactory when the ICR-prepared saline anti-D was used to verify Rh type among donors.Conclusion\ This method is simple and repeatable. It is less influenced by the concentration of IgG antibodies in sera. The storage life of the finished preparation is long.更多还原