【摘要】 通过对团头鲂 RAPD片段的克隆、Southern杂交筛选和 DNA序列分析 ,设计出 3对 PCR引物 :M1F/ M1R、M3 F/ M3 R和 M5 F/ M5 R.这 3对引物分别扩增出约 1.0 0、1.15、0 .5 6kb的 DNA主带 ;H ae 能将前两个片段酶解成两个几乎相等或相似的小片段 .经部分团头鲂群体和其它 12种鱼 DNA PCR验证 ,除三角鲂外 ,这 3对引物具有很强的团头鲂特异性 ,重复性 10 0 % ,可以用于除三角鲂以外的团头鲂的分子标记或分子鉴定 .更多还原

【Abstract】 We designed three sets of locus-specific PCR primers: M1F/M1R?M3F/M3R and M5F/M5R, through random amplified polymorphic DNA(RAPD) fragment cloning?southern blot selecting and DNA sequencing analysis. By the primer sets, amplification products showed three major DNA bands:1.0 kb(M1F/M1R)?1.15 kb(M3F/M3R) and 0.5 kb(M5F/M5R). The former two can be digested into two almost same or similar fragments with HaeⅢ. The PCR results of all tested samples (n=30) and other 11 specieses of fish revealed the three sets of PCR primers are very special and highly reproductive (100%) for Megalobrama amblycephala Yih. Although the PCR results between Megalobrama amblycephala Yih. and Megalobrama terminalis (Rich.) are identity, the three PCR primer sets can be used as powerful tools for the identification and nuclear DNA markers of Megalobrama amblycephala Yih. except Megalobrama temindis (Rich.).更多还原