【摘要】 以雌、雄黄颡鱼 DNA池为模板 ,从 42 2个引物中筛选出 6 1个 RAPD反应重复性好、带型清晰明亮的引物 ,这些引物可用于黄颡鱼的分子标记或鉴定 .采用 10～ 11个引物先后对两批黄颡鱼 (简称群体 A和群体 B)进行群体 RAPD多样性分析 .在群体 A(2 1尾 )中共检测到 95个位点 ,其中 2 7个为多态位点占总位点数2 8.42 % ,其群体 Nei& L i氏遗传相似率为 90 .6 7% ,Shannon信息指数为 0 .0 918比特 ;在群体 B中共检出 46个位点 ,其中多态位点 12个占 2 6 .0 9% ,其群体 Nei& L i氏遗传相似率为 93.2 7% ,Shannon信息指数为 0 .0 6 70比特 .黄颡鱼的遗传多样性比已报道的普通鲫鱼和野生稀有鲫的低而高于长江鳙鱼更多还原

【Abstract】 primers whose RAPD reactions are reproductive and with clean bands are selected from 422 primers by using the DNA pools of both male and female Pelteobagrus fulvidraco as templates. These 61 primers can be used as molecular marker for the species identification. We apply 10\|11 primers to proceed RAPD analysis consequently to two groups. In the first group, 95 sites (bands) are revealed, 27 of which are polymorplic, upon 28.42%; the Nei&Li’s genetic similarity within population is 90.67% and Shannon’s information index is 0.091 8 bit. In the second group, 46 sites(bands) are revealed, 12 of which are polymorphic, upon 26.09%; the Nei & Li’s genetic similarity is 93.27% and Shannon’s information index is 0.067 0 bit. The genetic diversity of Pelteabagrus fulvidraco is lower than that of reported Carassius auratus L. and Gobiocypris rarus and higher than Aristichthys nobilis .更多还原