【摘要】 为建立一种有效诱导及克隆人类白细胞抗原 ( HL A) -抗原肽复合物特异性细胞毒性 T细胞 ( CTL)的方法。用 EB病毒转化的 B淋巴母样细胞系细胞作为刺激细胞 ,采用长期混合淋巴细胞培养法诱导自身外周血 T细胞库中抗原肽 - HL A复合物特异性 CTL 增殖、分化 ,并用有限稀释法对之进行克隆。通过细胞毒实验证实获得的 CD3 +、CD8+ T细胞克隆具有高度抗原特异性并受某些 HL A型别的限制。更多还原

【Abstract】 In order to establish an effective method to induce and clone HLA-antigen peptide specific cytotoxic T lymphocyte (CTL), Epstein-Barr-virus-transformed B lymphoblastoid cell lines (EBV-LCLs) were used as stimulators to induce the proliferation and differentiation of the HLA-antigen peptide specific CTL in peripheral blood lymphocytes by long-term mixed lymphocyte culture. The CTLs were cloned using limiting dilution method. The obtained CD3 + and CD8 + T clones showed high specific towards antigen and their cytotoxic activity were restricted by some HLA phenotypes.更多还原