【摘要】 探讨丙型肝炎病毒 ( HCV)准种在 NS2区的基因结构特征。利用逆转录 -巢式 PCR从一份 HCV慢性携带者的阳性血清及一份急性丙肝患者的血清中获得 HCV NS2全长 c DNA,将其克隆于 T载体 ,各随机挑取 5个阳性克隆进行序列测定。结果显示在克隆到的 HCV NS2全长基因中 ,慢性携带者该区序列有一个于 HCV多聚蛋白第 83 5位氨基酸的位置出现终止信号 ,丙肝患者该区序列有三个于第 83 5位氨基酸的位置出现终止信号 ,一个小于第 887位氨基酸的位置出现终止信号。在克隆到的 HCV NS2基因中存在多个 N端终止突变序列 ,提示 NS2 N端跨膜区可能与包膜蛋白区有着密切的联系更多还原

【Abstract】 To explore the genetic variation characteristics of nonstructural (NS)2 quasispecies of hepatitis C virus, NS2 genes of HCV from a HCV asymptomatic carrier and a patient with abnormal ALT level were amplified by RT-nested-PCR and cloned into T vector. Five positive clones from each of them were selected randomly for sequencing. The results showed that one of the NS2 gene clones from the asymptomatic carrier had an in-frame stop codon at 835aa of HCV polyprotein. When it came to the patient with abnormal ALT level, 3 clones had stop codons at 835aa and 1 clone had a stop codon at 887aa of HCV polyprotein because of variation. Several N terminal stop codons in NS2 region of hepatitis C virus suggested that N-terminus of NS2 had close relation to envelop protein regions.更多还原