【摘要】 为研究癌基因 c- m yc和 n- ras在妊娠滋养细胞疾病 (GTD)发生、发展中的作用 ,用地高辛标记的 c- myc、n- ras裸核探针与 5 4例石蜡包埋的 GTD组织进行原位杂交。结果显示 :c- m yc m RNA在葡萄胎、侵蚀性葡萄胎和绒癌组织中阳性表达率分别为 44 .4%、 5 5 .6 %和 83.3% ,绒癌 c- myc阳性表达率高于葡萄胎 (P<0 .0 5 )。n- ras m RNA在葡萄胎、侵蚀性葡萄胎和绒癌组织中阳性表达率分别为 88.9%、 77.8%和 44 .4% ,绒癌 n- ras阳性表达率显著低于葡萄胎(P<0 .0 1)和侵蚀性葡萄胎 (P<0 .0 5 )。 ～ 期妊娠滋养细胞瘤 (GTT)组织中 c- m yc阳性表达率为 90 .9% ,高于 期的 45 .5 % (P<0 .0 5 )。c- m yc和 n- ras基因在 GTT组织中共同表达率为 47.2 %。结果提示 :c- myc基因与 GTD病变的发展及 GTT临床分期有关 ,N- ras基因可能为 GTT发生的早期事件 ,c- myc和 n- ras基因在 GTD癌变过程中起协同作用更多还原

【Abstract】 To study the role of c myc and n ras oncogenes in the development of gestational trophoblastic disease (GTD), in situ hybridization with digoxigenin labeled oligonucleotide probe was used to detect the expression of c myc and n ras genes in the paraffin embedded GTD tissues in 54 cases of GTD. The expression rate of c myc mRNA in hydatidiform mole (HM), invasive mole (IM) and choriocarcinoma (CC) was 44.4 %, 55.5 % and 84.4 % respectively. The expression rate of c myc in CC was significantly higher than that in HM ( P <0.05). The positive rate of n ras in HM, IM and CC was 88.9 %, 77.8 % and 44.4 % respectively. The positive rate of n ras in CC was significantly lower than that in HM ( P <0.01) and in IM ( P <0.05). The expression rate of c myc in stage Ⅲ IV of gestational trophoblastic tumor (GTT) was 90.9 % and 45.5 % in stage I respectively ( P <0.05). The co expression rate of c myc and n ras mRHA in GTT was 47.2 %. c myc oncogene is related to the progress of GTD and the clinical stage of GTT; n ras oncogene is an early event of GTT. c myc and n ras multioncogenes act synergetically in the progress of GTD.更多还原