【摘要】 目的 :探讨氟铝联合对体外培养人胚成骨细胞生长发育的影响。方法 :采用无血清体外细胞培养方法 ,将成骨细胞分为空白对照组、低铝组、高铝组、低铝低氟组、高铝低氟组、高铝高氟组共6组,观察氟铝共存对其生长发育的影响。结果 :加入氟铝后第14天和第16天 ,高铝组及高铝低氟组的细胞存活数均显著低于对照组、低铝组和低铝低氟组 (P<0.05)。高铝组的细胞变化坏死率明显高于对照组、低铝组及低铝低氟组 (P<0.05) ,高铝组加入不同剂量氟可抑制细胞的变性坏死率 ,但仍明显高于对照组、低铝组和低铝低氟组 (P<0.05) ,而低于高铝组 (P<0.05)。结论 :高剂量铝可抑制体外培养的人胚成骨细胞的生长发育并对其产生一定的毒作用 ,在高铝情况下 ,加入一定量氟可抑制铝对成骨细胞的毒作用。更多还原

【Abstract】 Objective: To study the effect of aluminum and fluorine on the growth and development of human embryonic osteoblasts in culture. Methods: Using the serum-free medium cell culture in vitro, the cultured osteoblasts were divided into six groups: control, low dose Al, high dose Al, low dose Al-F, high dose Al and low dose F group, high dose Al-F group, and the growth and development of osteoblasts were observed. Results: The amount of survival cells in high dose Al and high dose Al-F groups were lower than those in control, low dose Al and low dose Al-F groups significantly (P<0.05) at 14th day and 16th day after Al and F were added. The detection rate of cell degeneration and necrosis in high dose Al group was higher than that in control, low dose Al and low dose Al-F groups significantly (P<0.05). The detection rate of cell degeneration and necrosis in high dose Al with different dose F groups could be inhibited, but it still was higher than that in control, low dose Al and low dose Al-F groups significantly (P<0.05) and was lower than that in high dose Al group (P<0.05). Conclusion: High dose Al could inhibit the growth and development of human embryonic osteoblasts in culture in vitro and had a certain degrec of toxicity to them. with high dose Al, adding certain dose of F could inhibit the cell toxicity of Al to osteoblasts.更多还原