【摘要】 利用绿僵菌 Metarrhizium anisopliae的发酵过程研究了菌丝体形态与菌体生长的关系 .与菌体量特征相似 ,菌丝体形态特征也与菌体生长阶段相对应 :菌体形态图像的分形特征在生长迟缓期后期和对数生长期前期呈上升趋势 ,在对数生长期中期达最大值 2 .4.这可能是由于粗糙型弹丸状菌丝体 (rough pellet)与光滑型弹丸状菌丝体 (smooth pellet)比率在菌体生长过程中的显著变化引起的 .而菌丝体形态图像的分形分析可以很好地表征这种变化 .另一方面 ,菌丝体形态还与菌体的生物转化活性相关联 :在甾体底物 (16α,17α-环氧黄体酮 )加入发酵液进行生物转化之前 ,取样分析菌体形态分形特征 ,结果表明菌体较高的分形特征值与菌体较高的甾体转化率相对应 .同时发现菌体分形维数的最大值先于菌体量的最大值出现 ,表明与利用菌体量曲线来确定发酵工艺的传统方法相比 ,利用分形形态曲线可以更及时有效地指导发酵工艺的确定更多还原

【Abstract】 The fermentation with mold metarrhizium anisopliae has been carried out to determine the relationship between mycelial morphology and growth.Like the biomass concentration,the mycelial morphology seems to be coupled to growth phases:the fractal dimension,a feature calculated from the morphological images,increases during the late lag phase and the early exponential phase,and culminates at a value near 2.4 at half of the exponential phase.This can be attributed to the marked change of rough and smooth pellet proportions during growth and the change can be well expressed by the fractal analysis of mycelial morphology.The mycelial morphology is also strongly related to the biotransformation activity:a mycelial sample was withdrawn from the culture to use for fractal analysis before the steroid substrate (16α,17α epoxy 4 pregnene 3,20 dione) was added;the higher fractal dimension corresponds to the higher conversion rate of steroid biotransformation.And the maximum of fractal dimension appeared prior to the maximum of biomass concentration.It is therefore possible to utilize the fractal dimension curve to supervise the fermentation process more timely and availably than to use the conventional biomass curve to do.更多还原