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分光光度法测定不同时期半边旗总黄酮含量

Determination of Contents of Total Flavonoids in Pteris semipinnata L.in Different Periods

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【作者】 傅玉萍苟占平胡菊英吴铁

【Author】 FU Yu-ping 1, GOU Zhan-ping 2, HU Ju-ying 3, WU Tie 1 (1.Medical Science Developing Centre of Guangdong Medical College, Zhanjiang Guangdong 524023, China; 2.Laboratory for Reserch and Development of Natural Drugs, Guangdong Medical College, Zhanjia

【机构】 广东医学院医药科技开发中心广东医学院广东天然药物研究与开发实验室甘肃省天水市妇幼保健院广东医学院医药科技开发中心 广东湛江524023广东湛江524023741000广东湛江524023

【摘要】 目的 :为合理利用半边旗药材资源提供参考与启示。方法 :以芦丁为对照品 ,采用分光光度法在 5 0 0 nm处测定半边旗总黄酮含量。结果 :对照品在 2~ 2 0μg/m l范围内线形关系良好 ( r=0 .9994) ,平均回收率为 99.3%,RSD=1.12 %( n=6 )。结论 :该方法简便 ,准确 ,稳定 ,灵敏 ,重现性好 ,可作为半边旗的合理用药、质量控制及进一步进行其黄酮类成分研究的参考。

【Abstract】 Objective:To provide reference and inspiration for reasonable using medicinal herb of Pteris semipinnata L. Methods:A method of spectrophotometry was used in determining the flavonoids of Pteris semipinnata L. at wavelength of 500 nm. Results:The linear range was 2~20 μg/ml, r=0.999 4, the average recovery was 99.3% and RSD was 1.12% (n=6). Conclusions:The method is simple, accurate, stable, sensitive and has a good reproducibility, it can be used as a reference for quality control and reasonable medication as well as further study on the flavonoids constituents of Pteris semipinnata L..

  • 【文献出处】 时珍国医国药 ,LiShiZhen Medicine and Materia Medica Research , 编辑部邮箱 ,2001年12期
  • 【分类号】R284.2
  • 【被引频次】11
  • 【下载频次】201
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