【摘要】 利用改良的培养基 纤维蛋白平板法 ,从我国传统食品豆豉中筛选出具有高纤溶活性的纳豆杆菌菌株 ;菌株液体发酵法制得粗酶液 ,采用沉淀法及柱层析等方法从发酵液中初步分离纳豆激酶 ;并应用体外溶栓实验考察分析了纳豆激酶的纤溶活性 ,为开发新型溶栓药物及保健食品提供了实验参考依据更多还原

【Abstract】 Two Bacillus subtilis strains with high fibrinolysis activity were screened from traditional Chinese food Douchi with an improved medium fibrinogen plate. A study and optimization on the fermentation process, nattokinase has been separated from the fermented fluid with precipitation and column chromatography. The fibrinolysis activity of nattokinase was determined and analyzed in the thrombolysis experiment in vitro . The method will provide experimental evidence for developing new thrombolytic medicine and health food.更多还原