【摘要】 目的　建立外周血树突状细胞 (DendriticCell,DC)的分离和培养方法 ,研究DC对T细胞的刺激效应。方法　首先从外周血分离出非T细胞 ,再经贴壁和Metrizamide分离纯化去除巨嗜细胞、单核细胞及B细胞等 ,加入GM -CSF、TNF -α和IL - 4培养。收获DC与异体T细胞混合培养。用MTT法检测T细胞的增殖 ,ELISA法测定培养上清中IL - 2、IL - 6及IFN -γ的分泌量。结果　来源于外周血的DC前体细胞经细胞因子诱导培养后 ,其总量可扩增 18.34± 6 .2 6倍 ,可促进异体T细胞的增殖及细胞因子分泌。结论　来源于外周血的DC ,其数量和浓度均较高 ,而且是一种功能性DC。更多还原

【Abstract】 Objective To establish the method of inducing dendritic cells (DCs) from peripheral blood and analysis of their effects on T cells.Method The procursors of dendritic cells isolated from peripheral blood were induced with cytokines (GM-CSF,IL-4 and TNF-α).The proliferation of T cells was analysed by MTT method,and the concentration of cytokines (IL-2,IL-6 and IFN-γ) in T cells curture supernatant was detected by ELISA.Results The dendritic cells could expand and stimulate T cells secreting the cytokines.Conclusion The dendritic cells from peripheral blood shows a high quantity of production,and are functional.更多还原